The Orthology Benchmarking Service is an automated web-based service to facilitate orthology benchmarking, established and maintained by the Quest for Orthologs community.
Users of the orthology benchmarking web service can take advantage of two main functionalities, accessible from the landing page:
Several well-established orthology tools have run their methods on a Reference Proteome Set and have already been benchmarked. Their results are publicly available online for comparison. On the homepage (depicted in Figure 1), click on “Public Results” and select which Reference Proteome Set to view from the drop down menu.
On this page, the publicly-available benchmarking results are displayed, organized by type of benchmark.
Select a Benchmark from one of the two categories. For the Species Tree Discordance and Generalized Species Tree Discordance Benchmarks, it is necessary to choose a taxonomic group (displayed as tabs below).
In the Generalized Species Tree Discordance Benchmark example, clicking on the Vertebrate tab displays a plot showing the publicly-available results of the benchmark.
In all benchmarking result plots, the Precision versus Recall curve is shown. An arrow is displayed which points to the better predictions. The Pareto frontier is shown as a dashed line. Additionally, one can classify the results based on square quartiles, diagonal quartiles, or K-means clustering.
This function is for users who wish to run all the standard benchmarks on their own orthology inferences. One key point is that to avoid mapping issues and biases due to differences in input data, orthology inferences must be made on one of the Quest for Orthologs reference proteome sets. In that way, the results will be directly comparable to other publicly-available methods.
The overall workflow for using the benchmark service is as follows:
In order to compare different orthology prediction methods, a common set of protein sequences must be used. For this purpose, the QfO community, in collaboration with the EBI, has established the Reference Proteome set of proteomes. These proteomes have been selected to cover well-studied model organisms and other organisms of interest for biomedical research and phylogeny.
The reference proteomes are released yearly, in April. The best practice for new methods is to run on the reference proteome dataset of the previous year. For instance, in June 2020, a user will typically perform their analyses on the 2019 dataset. In that way, there will already be some points of comparisons. Established methods are encouraged to submit predictions on the latest dataset.
Reference proteomes for various years are available here.
First, prepare your ortholog predictions to upload in one of the acceptable file formats. Our benchmarks currently assess orthology on the basis of protein pairs. Therefore, we ask our users to upload their prediction in a format from which we can extract pairwise relations in an unambiguous manner. We support:
For both formats, we expect you to submit your predictions in a single file. This file might also be compressed by gzip or bzip2. In that case, it needs to have the proper filename extension (.gz or .bz2).
Next, from the homepage, click on Submit Predictions. This redirects to the QfO page on OpenEBench:
On the OpenEBench page, you are able to upload your orthology predictions. You can either create a user account to save your workspace, or run as a temporary account (limited to 2 Gb and active for one week). If you already have an account, you can select your project; otherwise you can create one.
Upload the ortholog predictions by using the left drop-down menu: Get Data -> Upload Files, and select your files to upload.
Your ortholog inferences (in one of the acceptable formats, described above) must be loaded into the workspace. This is done by clicking Select, under Tool settings -> File inputs -> Ortholog predictions to evaluate. If this is your first time, you will need to follow the instructions under +Can’t find your data?
As an example, we import a dataset by choosing Get Data -> Import Example Dataset -> and selecting QfO challenge 6 for the supplied datasets. The data includes the benchmarking metrics computed for two different sets of ortholog pairs:
After uploading the data into your workspace, you can select the file (click on the left-hand side box), then select the QFO Challenge 6 from the Action drop-down menu on the right-hand side.
After selecting your orthology data, you can choose which benchmarks to run underneath Settings -> Benchmarking Challenges Identifiers. In this example we run the Gene Ontology conservation test and the Agreement with Reference Gene Phylogenies: SwissTree only.
After clicking submit, the job will be sent to the OpenEBench servers for computation. The job can be monitored from the Project Workspace, under Last Jobs.